DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
|• L-glutamine||• Glucose|
|• Phenol Red||• Sodium Pyruvate|
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture DMEM at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
|Phenol Red Indicator:||Phenol Red|
|Serum Supplementation:||Standard Serum Supplementation|
|HEPES Buffer:||No HEPES|
|Sodium Pyruvate Additive:||No Sodium Pyruvate|
|Product Size:||500 mL|
|Volume (Metric):||500 ml|
|Green Features:||Sustainable packaging|
|Shipping Condition:||Room Temperature|
Contents & storage
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture