DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
|• L-glutamine||• HEPES|
|• Phenol Red|
The complete formulation is available.
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (11320-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
|Phenol Red Indicator:||Phenol Red|
|HEPES Buffer:||No HEPES|
|Sodium Pyruvate Additive:||Sodium Pyruvate|
|Serum Supplementation:||Standard Serum Supplementation|
|Product Size:||500 mL|
|Volume (Metric):||500 ml|
|Green Features:||Sustainable packaging|
|Shipping Condition:||Room Temperature|