The XLarge DNA Ladder RTU is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, which is premixed with loading buffer dye for direct loading on gel.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1mM EDTA.
Range: 250-25K bp
Number of bands: 14
Concentration: 100 μg/ml
Recommended Load: 5 μl / well
Containing orange G & xylene cyanol FF as tracking dyes.
Store at 25°C for 6 months
Store at -20°C for 24 months