Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA and shRNA-based gene knockdown experiments, as well as gene expression studies. Lipofectamine® 2000 is the best reagent available from Life Technologies for the co-transfection of siRNA and plasmid DNA.
With Lipofectamine® 2000 Reagent you will get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (Table 1)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum – eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A High-Performance Transfection Reagent for Gene Expression and Gene Silencing
Lipofectamine® 2000 Reagent works effectively with all common cell lines as well as many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine® 2000 Reagent’s high-efficiency transfections provide the high levels of gene knockdown needed to achieve convincing results. Lipofectamine® 2000 Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine® 2000 Reagent is easy to use – simply mix with nucleic acid and add to cell culture.
Ideal for High-Throughput Work
Simplicity and speed combined with high transfection efficiency make Lipofectamine® 2000 Reagent ideal for transient protein expression or high throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications. Protocols, citations, and additional product information for Lipofectamine® 2000 can be found in the Cell Lines Database.
|Cell Type:||Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells|
|Sample Type (Specific):||Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA|
|Transfection Technique:||Lipid-Based Transfection|
|High Throughput Compatibility:||High Throughput-Compatible|
|Product Size:||1.5mL、0.75 mL|
|Shipping Condition:||Approved for shipment at Room Temperature or on Wet Ice|
|Green Features:||Sustainable packaging|