Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
|• Phenol Red||• Ribonucleosides|
|• L-glutamine||• Deoxyribonucleosides|
|• Sodium Bicarbonate|
The complete formulation is available.
Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® MEM α product made in our Scotland facility (12000-014). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
|Phenol Red Indicator:||Phenol Red|
|HEPES Buffer:||No HEPES|
|Sodium Pyruvate Additive:||Sodium Pyruvate|
|Product Size:||10 x 1 L|
|Serum Supplementation:||Standard Serum Supplementation|
|Shipping Condition:||Room Temperature|
Contents & storage
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture